Journal: International Journal of Molecular Sciences

Article Title: Liposomal Encapsulation of Citicoline for Ocular Drug Delivery

doi: 10.3390/ijms242316864

Figure Lengend Snippet: Size distribution with polydispersity index (PDI) and ζ-potential for all synthesized liposomes. The values are the average of three measurements (mean ± esd).

Article Snippet: An amount of 200 μL of liposomes containing citicoline as first placed in Amicon centrifuge tubes (cutoff 3 kDa), then 800 μL of physiological fluid obtained by emulsion oil/water was added to the tube and centrifuged at 5500 rpm for 1 h in a refrigerated centrifuge at 277 K (Thermo Scientific SL 16R, Monza, Italy).

Techniques: Synthesized, Liposomes, Concentration Assay

Journal: International Journal of Molecular Sciences

Article Title: Liposomal Encapsulation of Citicoline for Ocular Drug Delivery

doi: 10.3390/ijms242316864

Figure Lengend Snippet: Release of citicoline over 6 h in simulated ophthalmic fluid and first-order fitting data The R 2 coefficient is 0.998 and 0.999 for DOPC:Chol, Cit (1:1), 1 and DOPC:Chol, Cit (2:1), 1, respectively.

Article Snippet: An amount of 200 μL of liposomes containing citicoline as first placed in Amicon centrifuge tubes (cutoff 3 kDa), then 800 μL of physiological fluid obtained by emulsion oil/water was added to the tube and centrifuged at 5500 rpm for 1 h in a refrigerated centrifuge at 277 K (Thermo Scientific SL 16R, Monza, Italy).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Liposomal Encapsulation of Citicoline for Ocular Drug Delivery

doi: 10.3390/ijms242316864

Figure Lengend Snippet: 1 H-NMR spectrum of citicoline 10 −2 M in D 2 O with proton assignment recorded at 600 MHz and 298.15 K. Chemical structure of citicoline (cytidine-5′-diphosphocholine) with numbering is also reported.

Article Snippet: An amount of 200 μL of liposomes containing citicoline as first placed in Amicon centrifuge tubes (cutoff 3 kDa), then 800 μL of physiological fluid obtained by emulsion oil/water was added to the tube and centrifuged at 5500 rpm for 1 h in a refrigerated centrifuge at 277 K (Thermo Scientific SL 16R, Monza, Italy).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Liposomal Encapsulation of Citicoline for Ocular Drug Delivery

doi: 10.3390/ijms242316864

Figure Lengend Snippet: 1 H NMR peak assignments of citicoline in D 2 O. The multiplicity is also reported.

Article Snippet: An amount of 200 μL of liposomes containing citicoline as first placed in Amicon centrifuge tubes (cutoff 3 kDa), then 800 μL of physiological fluid obtained by emulsion oil/water was added to the tube and centrifuged at 5500 rpm for 1 h in a refrigerated centrifuge at 277 K (Thermo Scientific SL 16R, Monza, Italy).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Liposomal Encapsulation of Citicoline for Ocular Drug Delivery

doi: 10.3390/ijms242316864

Figure Lengend Snippet: 1 H-NMR spectra of liposomes: ( a ) DOPC: Chol, Cit (2:1), 1; ( b ) DOPC:Chol (2:1); and ( c ) citicoline 10 −2 M in D 2 O recorded at 600 MHz and 298.15 K.

Article Snippet: An amount of 200 μL of liposomes containing citicoline as first placed in Amicon centrifuge tubes (cutoff 3 kDa), then 800 μL of physiological fluid obtained by emulsion oil/water was added to the tube and centrifuged at 5500 rpm for 1 h in a refrigerated centrifuge at 277 K (Thermo Scientific SL 16R, Monza, Italy).

Techniques: Liposomes

Journal: International Journal of Molecular Sciences

Article Title: Liposomal Encapsulation of Citicoline for Ocular Drug Delivery

doi: 10.3390/ijms242316864

Figure Lengend Snippet: FTIR-ATR spectrum for ( a ) stacked and ( b ) overlapped (1700–750 cm –1 ) DOPC:Chol (1:1) (black); (DOPC: Chol), Cit (1:1), 1 (red); and citicoline (blue).

Article Snippet: An amount of 200 μL of liposomes containing citicoline as first placed in Amicon centrifuge tubes (cutoff 3 kDa), then 800 μL of physiological fluid obtained by emulsion oil/water was added to the tube and centrifuged at 5500 rpm for 1 h in a refrigerated centrifuge at 277 K (Thermo Scientific SL 16R, Monza, Italy).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Liposomal Encapsulation of Citicoline for Ocular Drug Delivery

doi: 10.3390/ijms242316864

Figure Lengend Snippet: FTIR-ATR spectrum for ( a ) stacked and ( b ) overlapped (1700–750 cm –1 ) DOPC:Chol (2:1) (black); (DOPC: Chol), Cit (2:1), 1 (red); and citicoline (blue).

Article Snippet: An amount of 200 μL of liposomes containing citicoline as first placed in Amicon centrifuge tubes (cutoff 3 kDa), then 800 μL of physiological fluid obtained by emulsion oil/water was added to the tube and centrifuged at 5500 rpm for 1 h in a refrigerated centrifuge at 277 K (Thermo Scientific SL 16R, Monza, Italy).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Liposomal Encapsulation of Citicoline for Ocular Drug Delivery

doi: 10.3390/ijms242316864

Figure Lengend Snippet: Main bands and assignment observed in FTIR spectra of plain liposomes and citicoline.

Article Snippet: An amount of 200 μL of liposomes containing citicoline as first placed in Amicon centrifuge tubes (cutoff 3 kDa), then 800 μL of physiological fluid obtained by emulsion oil/water was added to the tube and centrifuged at 5500 rpm for 1 h in a refrigerated centrifuge at 277 K (Thermo Scientific SL 16R, Monza, Italy).

Techniques: Liposomes

Journal: International Journal of Molecular Sciences

Article Title: Liposomal Encapsulation of Citicoline for Ocular Drug Delivery

doi: 10.3390/ijms242316864

Figure Lengend Snippet: ( a ) TOF-SIMS positive ion spectra of citicoline ( m / z range 100–400); ( b ) structure and characteristic fragments of citicoline.

Article Snippet: An amount of 200 μL of liposomes containing citicoline as first placed in Amicon centrifuge tubes (cutoff 3 kDa), then 800 μL of physiological fluid obtained by emulsion oil/water was added to the tube and centrifuged at 5500 rpm for 1 h in a refrigerated centrifuge at 277 K (Thermo Scientific SL 16R, Monza, Italy).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Liposomal Encapsulation of Citicoline for Ocular Drug Delivery

doi: 10.3390/ijms242316864

Figure Lengend Snippet: ( a ) Hydrodynamic size of plain and citicoline-loaded liposomes, and ( b ) mean PDI values of plain and citicoline-loaded liposomes over three months at 4 °C (average error 5%).

Article Snippet: An amount of 200 μL of liposomes containing citicoline as first placed in Amicon centrifuge tubes (cutoff 3 kDa), then 800 μL of physiological fluid obtained by emulsion oil/water was added to the tube and centrifuged at 5500 rpm for 1 h in a refrigerated centrifuge at 277 K (Thermo Scientific SL 16R, Monza, Italy).

Techniques: Liposomes